/myco/ - Mycology

Some improvements to this that I want to include on a rewrite:

>bucket pasteurization

Can often sterilize rather than pasteurize, leaving you exposed to contaminants taking hold of your substrate before the mycellium can fully colonize. Either a proper pasteurization or a more detailed bucket tek needs to be included.

>spawning the tub

A note on leaving a small amount of substrate, unmixed with the spawn, should be added to this tek for something to cover the exposed rye berries / kernels with. Otherwise, again, you run the risk of contaminants taking hold, this time of the exposed berries.

Did enjoy the tek and the effort you put into though. I was heartbroken last night to come back from a 3 day trip expecting pins, only to find green mold had begun colonizing my tub. Just constructive criticism for other noobies like me following this tek :-)

>>114

How trippy

Just letting you know that you have another reader on this board!

According to Wikipedia, this entire site was conceptualized while hot wheels was tripping balls on mushies.

>the more you know!

>>78

BUMP

>>259

whoa HELLO

nice conk

I see the first pic all the time around my house. second one is a qt3.14. 3rd one is called conk around here. fourth pic looks like some sort of boletaceae ?

>>250

From what I'm finding it seems the standard DIY method is to just stuff the neck of the bottle with polyfill, then put tin foil over it. Just seemed to ez! I'll be back with results l8r, mycofags

Watch the agar section in the grow video sticky.

>>254

Ended up doing like RR said and cutting up a SFD (synthetic filter disc) and using a nail to make a hole in the lid. Gonna sterilize my agar today and work with it for the first time.

>>253

Why is the foil below the lids here? Or am I misinterpreting?

What's an SAB? Can't find anything online.

Do any of you guys have specific jar types that you like to use?

Still trying to see if there is a "beginner" kit out there. I'm going to experiment on my own for a while on the cheap, but I'd also like to know where I can get the stuff and a small list for when I will need it.

>>205

Still air box.

The jar you use depends on the substrate you use. If its not crumbly, like brown rice flour for PF cakes, then you need a wide mouth jar so that the whole substrate can slide right out. If its easily separable, like rye berries, then it doesn't matter.

Fuck a kit,watch the first video in the lets grow mushrooms video in the sticky.

>>214

This. Kits are a scam homie g

>>195

>>196

>>197

One way to make agar plates last a little longer is to wrap parafilm around the edges of the plate and stack them upside down so moisture droplets don't fall on your pure culture. Don't know if this has been suggested yet.

>>229

I sterilize my agar in one and pour from it.

Check out the keys grow mushrooms video thread.RR uses a whiskey bottle but I like the carpenter better.fungi perfecti has them

>>237

>whiskey bottle

I've heard I can use mason jars.. On the off chance that someone will respond before I end up digging up the info myself (currently looking), what's an easy tek guide for making an agar flask? Can I mason jar with the rubber side up work?

>>238

dug this gem up

http://www.shroomery.org/forums/showflat.php/Number/13450919

>>237

Fuck my phone and it's misspelling bullshit.

You can use a mason jar but I've had more luck with an erelenmyer.

>>245

So how exactly do I convert a whisky bottle into an agar flask? I've been looking online for awhile now and can't seem to find anything. The only bottles I have are corked, and I'd think I'd need a metal lid.

>>226

Well the idea of a flow hood is to create a sterile, still air space for you to work in. Which in something like grain spawn is unnecessary as you make a sterile "habitat" inside of your jars after you pressure cook them.

A flow hood comes in handy if you plan on doing g2g grain to grain transfers. As you'll have to open your jars exposing the sterile habitat you created during pressure cooking, to open air and whatever contams are floating in it. But with a flow hood the air inside should be sterile or atleast more so than the air outside of the flow hood making contams floating inside of your jars less likely.

So flow hoods come in handy whenever you need a sterile envirement to work in. Which with alot of methods nowadays don't require it. Grain spawn, both in jars and spawn bags, and I believe BFR cakes all work by making a sterile habitat in their respected container for your mycelium to get a grow and when using a syring as long as it has been properly sterilised should not infringe on that sterile habitat inside of the jar/bag or whatever.

>>232

Typo near the end hope you get what i what I meant

>>232

Okokok, I see. So there's nothing a flow hood can do that a SAB can't also accomplish?

>>240

Is there any point then in tech'ing up to a flow hood? I can make for under $100 and I've been considering adopting that as a new project.

>>241

No,I do agar in my SAB and everything is clean.

Watch the videos on the video thread.

Being sterile is more for spawn creation/agar.

Watch the videos man,it has everything but monotubs and the sticky has the monotub tek.

Yes. Took them twice (8g each time). I ate the caps straight, after they had been dried. Experienced two discrete sets of symptoms. Set 1 began roughly 45 minutes after ingestion and lasted for 2.5 hours. Set two began after I vomited a couple of times and persisted for 6 hours.

1. Dizziness, extreme nausea, vomiting, vertigo, flushed cheeks, lethargy

2. Buzzing sensations in ears, eyes, and on skin, combined with closed eye hallucinations, and disassociation. My brain was emptying all of its thoughts in front of my eyes stream-of-consciousness style. This kind of experience could be very troubling if the user is not familiar with meditation and letting go of thoughts/feelings.

The comedown was the opposite of an alcohol hangover. My mind was extremely clear and peaceful and I felt physically great for the next 12 hours.

I want to do them again but I can't bring myself to eat them because the taste/texture is now strongly linked to vomiting in my brain.

>>208

Where they prepared differently the two times?

>>209

you're a dope. read the post again.

>>211

Way to be an ass. From what I understand the preparation greatly reduces the negative effects, and since you had two distinctly different "trips", I wanted it to be clear whether or not they were prepared differently. Why the fuck would you ever eat amanita fresh?

>>225

I mean,if they were heat dried I guess that'd convert the ibotenic acid into muscimol, but if they were fan dried I don't know if it would..

Thanks for the report,Anon.

Sounds like you had a good time.

Are you interested in growing them?

Thanks again for the trip report anon

>>179

No problem. I might start growing them in the future, but any time soon I most likely won't be. Shrooms are the first psychedelics I've tried, so I hope to try acid next, but of the drugs I've tried, shrooms have definitely been my favourite

>>192

Make sure you get a test kit on amazon if you could across acid.

Lots of bullshit outthere being passed off as acid.

I buy from sporeworks but remember using spores can result in different batches everytime.

Thats whybyou want a clone or an isolate

OP here. This is going to sound stupid, but I will be attempting this with shiitake stem butts.

>>165

I know

OK, basically did this:

1. Got a screw-on mason jar, filled it with dry used coffee grounds (free from coffee shop)

2. I then put this coffee into a glass container, put plastic wrap over it, and microwaved it on high for 7 minutes. It was incredibly hot.

3. While everything was microwaving, I sterilized everything with 70% alcohol. This includes the knife I used for cutting, little bowls for holding the stem butts, a spoon, the mason jar, coffee filters, and a rubber band.

4. Once the coffee was done microwaving, I let it cool until it was warm to the touch. I then filled it up in increments by putting in a few spoonfuls at a time. Between spoonfuls, I would cut off the stem butts from the shiitake mushrooms and put a few between the "layers" of coffee grounds. I rupped some of them up a bit on purpose.

5. I realized the coffee grounds were a bit dry. I added like 10-20% of the weight of the coffee grounds in WATER that I had boiled in the microwave to it after it had cooled down to being warm.

6. I took TWO coffee filters and also sterilized and made them damp with rubbing alcohol ( isopropyl 70%, like before). I put them both over the mason jar and then screwed on the metal cap part of the mason jar (which was also sterilized).

7. I then fit a (sterilized) rubber band over the bottom to keep the coffee filters from flapping around.

8. I put the jar in my bathroom in the corner. This is a very clean bathroom.

Rate how stupid :D

>>161

Thank you for the link. Very insightful

Cap it and put it in a ziplock bag.zip locks are sterile until ypu open them so do it in a SAB.you can put the bag in a fridge and they will be goos for awhile.I've used spores I had in the fridge that were a year old.

Learn agar Anon,it's easy and worth it.

I posted the entire let's grow mushrooms dvd,check out the agar sections

>>97

Ty based anon. I'm just scared cuz I have so little left, one single contam and I could be out of an entire variety. Is agar just as sterile as any other method? I know with oysters, using coffee grounds makes it a much cleaner grow; things just don't like to grow in it, except oyster mycelium. I'm just looking for the most contam-proof, surefire method available.

I just made my SAB and I've been watching the vids you posted, I'm almost to the agar one. Great stuff, I had heard of the series before but always overlooked and undervalued it.

I'm curious, how exactly did you fuck it up ?

>>108

I just had loads of contaminants. My living situation was far from ideal, I was silly not t just wait for a better time.