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/sci/ - Science and Mathematics

Spending thousands of dollars on useless labs since 2014.

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Oh, hey. We're actually having old posts pruned now.

 No.2455

How hard would DIY genetic manipulation be?

Crisper is supposed to be easy as fuck but you need to do it on germ cells to get a second generation with the alterations throughout.

How hard is it to do IVF on mice? I don't care if I have to cut up some mice to get eggs and sperm.

The goal is to gain the skills required to make cat girls so I would want to genetically modify the mice with cat genes.

What would the base level of required equipment be?

 No.2458

>3d catgirls


 No.2466

Biologist working on CRISPR here.

>Crisper

If you can't even spell it, maybe you should find another hobby.

>How hard would DIY genetic manipulation be?

What exactly are you trying to manipulate? Introducing point mutations is pretty easy in flies or fish if you inject embryos. If you want mammals, good luck, even actual biologists hate making knockout mice without a very good reason (ie. Nature paper) because of how difficult it is.

For DIY, the big problems will be:

>figuring out how to sequence cheaply so you can confirm your manipulations

>obtaining dangerous chemicals like ems if doing mutagenesis

>budget is from $10k's to $1m's depending on scope

>ethics review for animal experiments

>storing samples without buying huge expensive -80C freezers and backup power system

>sterilizing shit without expensive autoclaves

Bacteria and other single cell organisms are doable, especially if you find a local hackspace or uni lab willing to accept volunteers.

>How hard is it to do IVF on mice?

Very hard. You can't do it on your own. Even with a PhD in relevant field it's hard. On your own, forget it.

>catgirls

Retarded idea, won't happen in your lifetime. Forget it.

If you are serious about this, change your major to Bio, get a Bio PhD from top 10 schools, learn Chinese and get a senior scientist job in China. You still won't be able to do it, but maybe a century after you're dead someone will do it based on the groundwork you laid down.

But who am I kidding, you can't even be bothered to Google the thing you want to work with to make sure you know how it's spelled. Good luck with your shit life.


 No.2467

>>2466

>For DIY, the big problems will be:

I should add, that is assuming you somehow have all the scientific wisdom of an experienced scientist without even going through a PhD program. In other words, it's just not gonna happen. Biology is hard, there's a reason why everyone doing it has a doctorate.


 No.3010

Spit on a plate, add nutrients for bacteria, slowly add anti-biotics. Genetic manipulation achieved.


 No.3034

>>3010

Already been done many times in olden papers.

Takes forever without a chemostat (difference between weeks and years), you need to at least use gamma rays or EMS.




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